pMPO 61
(Plasmid
#69869)
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Purpose(Empty Backbone) Expression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm- nasF attenuator-T7 SD sequence-MCSII, MCS M13 replication origin
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 69869 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepWSK29
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMPO 61 was a gift from Eduardo Santero (Addgene plasmid # 69869 ; http://n2t.net/addgene:69869 ; RRID:Addgene_69869) -
For your References section:
Improved expression systems for regulated expression in Salmonella infecting eukaryotic cells. Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E. PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 2011 Aug 1. 10.1371/journal.pone.0023055 PubMed 21829692