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Addgene

His-GIV-CTs
(Plasmid #69864)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69864 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28b
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 630
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GIV/Girdin
  • Alt name
    His-GIV-CTs
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    630
  • Mutation
    C terminal amino acids S1660-S1870 of human GIV.
  • Entrez Gene
    CCDC88A (a.k.a. APE, GIRDIN, GIV, GRDN, HkRP1, KIAA1212, PEHO, PEHOL)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde I (not destroyed)
  • 3′ cloning site EcoR I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His-GIV-CTs was a gift from Pradipta Ghosh (Addgene plasmid # 69864 ; http://n2t.net/addgene:69864 ; RRID:Addgene_69864)

  • For your References section:

    A GDI (AGS3) and a GEF (GIV) regulate autophagy by balancing G protein activity and growth factor signals. Garcia-Marcos M, Ear J, Farquhar MG, Ghosh P. Mol Biol Cell. 2011 Mar 1;22(5):673-86. doi: 10.1091/mbc.E10-08-0738. Epub 2011 Jan 5. 10.1091/mbc.E10-08-0738 PubMed 21209316
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