GST-GIV-CT
(Plasmid #69863)

• Purpose: Bacterial expression of GST tagged GIV-CT
• Depositing Lab: Pradipta Ghosh
• Publication: Garcia-Marcos et al J Biol Chem. 2010 Apr 23;285(17):12765-77. doi: 10.1074/jbc.M109.045161. Epub 2010 Feb 15.

Backbone

• Vector backbone: pGEX-4T-1
• Backbone size w/o insert (bp): 4969
• Total vector size (bp): 5709
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GIV/Girdin
• Alt name: GST-GIV-CT
• Species: H. sapiens (human)
• Insert Size (bp): 740
• Mutation: C terminal amino acids 1623-1870 of human GIV
• Entrez Gene: CCDC88A (a.k.a. APE, GIRDIN, GIV, GRDN, HkRP1, KIAA1212, PEHO, PEHOL)
• Promoter: TAC
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: Not I (not destroyed)
• 5′ sequencing primer: pGEX5
• 3′ sequencing primer: pGEX3

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry