Skip to main content
Addgene

pet28bZFCNGA5
(Plasmid #69858)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69858 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pet28b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5362
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZFCNGA5, 3' end only with native stop
  • Alt name
    CNGA5
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    324
  • GenBank ID
    DQ866815.1
  • Entrez Gene
    cnga5
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pet28bZFCNGA5 was a gift from Gary Matthews (Addgene plasmid # 69858 ; http://n2t.net/addgene:69858 ; RRID:Addgene_69858)

  • For your References section:

    A novel cyclic nucleotide-gated ion channel enriched in synaptic terminals of isotocin neurons in zebrafish brain and pituitary. Khan S, Perry C, Tetreault ML, Henry D, Trimmer JS, Zimmerman AL, Matthews G. Neuroscience. 2010 Jan 13;165(1):79-89. doi: 10.1016/j.neuroscience.2009.09.040. Epub 2009 Sep 22. 10.1016/j.neuroscience.2009.09.040 PubMed 19778592
Related items:
Commonly requested with: