POM43-mCherry
(Plasmid #69857)

• Purpose: N-terminal tagging with mCherry using LEU2 as a selection marker in S. cerevisiae.
• Depositing Lab: Liza Pon
• Publication: Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi: 10.1371/journal.pone.0146120. eCollection 2016.

Backbone

• Vector backbone: POM43
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 4756
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mCherry minus start and stop codon
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 701

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: N/A (use 3' primer)
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry