pFA6-mTFP1-His3MX6
(Plasmid
#69850)
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PurposeC-terminal tagging with mTFP1 using His3MX6 as a selection marker in S. cerevisiae.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69850 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFA6-GFP-His3MX6
- Backbone size w/o insert (bp) 4800
- Total vector size (bp) 5500
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Vector typeYeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemTFP1
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SpeciesClavularia sp.
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Insert Size (bp)711
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PacI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer Sp6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6-mTFP1-His3MX6 was a gift from Liza Pon (Addgene plasmid # 69850 ; http://n2t.net/addgene:69850 ; RRID:Addgene_69850) -
For your References section:
Characterization of Fluorescent Proteins for Three- and Four-Color Live-Cell Imaging in S. cerevisiae. Higuchi-Sanabria R, Garcia EJ, Tomoiaga D, Munteanu EL, Feinstein P, Pon LA. PLoS One. 2016 Jan 4;11(1):e0146120. doi: 10.1371/journal.pone.0146120. eCollection 2016. PONE-D-15-33334 [pii] PubMed 26727004
