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FUW TetO NET1A-V5 S46A
(Plasmid #69829)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69829 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUW TetO
  • Backbone size w/o insert (bp) 8431
  • Total vector size (bp) 10163
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Neuroepithelial cell-transforming gene 1 protein A
  • Alt name
    NET1A (short isoform of NET1), ARHGEF8
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1732
  • Mutation
    S46A
  • GenBank ID
    BC010285
  • Entrez Gene
    NET1 (a.k.a. ARHGEF8, NET1A)
  • Promoter minimal CMV promoter with tetracycline operator
  • Tag / Fusion Protein
    • v5 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site pac1 (not destroyed)
  • 3′ cloning site Nhe1 (not destroyed)
  • 5′ sequencing primer GTCAGATCGCCTGGAG
  • 3′ sequencing primer GACAACGGGCCACAACTCCTC or CAT AGC GTA AAA GGA GCA ACA TAG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Backbone is from Brambrink et al, 2008

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

cDNA is from the Orfeome library (Open Biosystems). Backbone is from Brambrink et al, 2008

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUW TetO NET1A-V5 S46A was a gift from Anne Brunet (Addgene plasmid # 69829 ; http://n2t.net/addgene:69829 ; RRID:Addgene_69829)

  • For your References section:

    Identification of AMPK Phosphorylation Sites Reveals a Network of Proteins Involved in Cell Invasion and Facilitates Large-Scale Substrate Prediction. Schaffer BE, Levin RS, Hertz NT, Maures TJ, Schoof ML, Hollstein PE, Benayoun BA, Banko MR, Shaw RJ, Shokat KM, Brunet A. Cell Metab. 2015 Nov 3;22(5):907-21. doi: 10.1016/j.cmet.2015.09.009. Epub 2015 Oct 8. 10.1016/j.cmet.2015.09.009 PubMed 26456332
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