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pECE M2-SH3PXD2A S974A
(Plasmid #69814)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69814 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pECE
  • Backbone size w/o insert (bp) 2898
  • Total vector size (bp) 6249
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SH3 and PX domain-containing protein 2A
  • Alt name
    SH3PXD2A, FISH, TKS5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3351
  • Entrez Gene
    SH3PXD2A (a.k.a. FISH, SH3MD1, TKS5)
  • Promoter SV40 early promoter
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 in backbone, Mfe1 in TKS5 pcr product (destroyed during cloning)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer CATTCTCCGCCCCATGGCTGAC
  • 3′ sequencing primer GTTTCAGGTTCAGGGGGAGGTG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    cDNA is from GeneCopoeia

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pECE M2-SH3PXD2A S974A was a gift from Anne Brunet (Addgene plasmid # 69814 ; http://n2t.net/addgene:69814 ; RRID:Addgene_69814)

  • For your References section:

    Identification of AMPK Phosphorylation Sites Reveals a Network of Proteins Involved in Cell Invasion and Facilitates Large-Scale Substrate Prediction. Schaffer BE, Levin RS, Hertz NT, Maures TJ, Schoof ML, Hollstein PE, Benayoun BA, Banko MR, Shaw RJ, Shokat KM, Brunet A. Cell Metab. 2015 Nov 3;22(5):907-21. doi: 10.1016/j.cmet.2015.09.009. Epub 2015 Oct 8. 10.1016/j.cmet.2015.09.009 PubMed 26456332
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