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Addgene

pSFFV-Cx45
(Plasmid #69808)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69808 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSFFV
  • Backbone manufacturer
    Fuhlbrigge, et al 1998, PMID 3261013
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cx45
  • Alt name
    GJC1
  • Alt name
    Connexin45
  • Species
    G. gallus (chicken)
  • Entrez Gene
    GJC1 (a.k.a. GJA7, GJD3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SFFV-F attgattgactgcccacctc
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was provided by Joseph Stains (University of Maryland School of Medicine), who received it from Thomas Steinberg (Washington University).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSFFV-Cx45 was a gift from Richard Veenstra (Addgene plasmid # 69808 ; http://n2t.net/addgene:69808 ; RRID:Addgene_69808)

  • For your References section:

    Multiple connexins confer distinct regulatory and conductance properties of gap junctions in developing heart. Veenstra RD, Wang HZ, Westphale EM, Beyer EC. Circ Res. 1992 Nov;71(5):1277-83. PubMed 1382884
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