Hp MTAN
(Plasmid
#69802)
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PurposeExpression of H. pylori J99 methylthioadenosine nucleosidase
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 69802 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJ414
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Backbone manufacturerDNA 2.0
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMethylthioadenosine nucleosidase
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Alt nameMTAN
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SpeciesH. pylori
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Insert Size (bp)700
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Tag
/ Fusion Protein
- Histidine tag (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Hp MTAN was a gift from Vern Schramm (Addgene plasmid # 69802 ; http://n2t.net/addgene:69802 ; RRID:Addgene_69802) -
For your References section:
A picomolar transition state analogue inhibitor of MTAN as a specific antibiotic for Helicobacter pylori. Wang S, Haapalainen AM, Yan F, Du Q, Tyler PC, Evans GB, Rinaldo-Matthis A, Brown RL, Norris GE, Almo SC, Schramm VL. Biochemistry. 2012 Sep 4;51(35):6892-4. doi: 10.1021/bi3009664. Epub 2012 Aug 22. 10.1021/bi3009664 PubMed 22891633