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Addgene

Hp MTAN
(Plasmid #69802)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69802 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pJ414
  • Backbone manufacturer
    DNA 2.0
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Methylthioadenosine nucleosidase
  • Alt name
    MTAN
  • Species
    H. pylori
  • Insert Size (bp)
    700
  • Tag / Fusion Protein
    • Histidine tag (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Hp MTAN was a gift from Vern Schramm (Addgene plasmid # 69802 ; http://n2t.net/addgene:69802 ; RRID:Addgene_69802)
  • For your References section:

    A picomolar transition state analogue inhibitor of MTAN as a specific antibiotic for Helicobacter pylori. Wang S, Haapalainen AM, Yan F, Du Q, Tyler PC, Evans GB, Rinaldo-Matthis A, Brown RL, Norris GE, Almo SC, Schramm VL. Biochemistry. 2012 Sep 4;51(35):6892-4. doi: 10.1021/bi3009664. Epub 2012 Aug 22. 10.1021/bi3009664 PubMed 22891633