pCR8/GW/TOPO BMI
(Plasmid
#69796)
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PurposeGateway entry vector for BMI
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69796 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCR8/GW/TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2817
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBMI
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Alt nameBMI1 proto-oncogene, polycomb ring finger
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SpeciesH. sapiens (human)
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Entrez GeneBMI1 (a.k.a. FLVI2/BMI1, PCGF4, RNF51, flvi-2/bmi-1)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCR8/GW/TOPO BMI was a gift from Gerard Cagney (Addgene plasmid # 69796 ; http://n2t.net/addgene:69796 ; RRID:Addgene_69796)