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Addgene

pUAST-HA, Pbl-A
(Plasmid #69792)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69792 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUAST-HA
  • Backbone manufacturer
    ?
  • Backbone size w/o insert (bp) 8900
  • Total vector size (bp) 11450
  • Modifications to backbone
    HA tag
  • Vector type
    Insect Expression ; P element-based pUAST vector for Gal4-regulated expression in Drosophila.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    LB media
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Pebble - Drosophila guanine nucleotide exchange factor
  • Alt name
    Pbl, Pbl-2A
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    2559
  • Mutation
    Encodes Pbl (NP_729306.1) lacking last 9 amino acids.
  • Promoter hsp70 promoter
  • Tag / Fusion Protein
    • HA tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ? (unknown if destroyed)
  • 3′ cloning site ? (unknown if destroyed)
  • 5′ sequencing primer pUAST for
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Sabine Schumacher, Alumni

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUAST-HA, Pbl-A was a gift from Arno Muller (Addgene plasmid # 69792 ; http://n2t.net/addgene:69792 ; RRID:Addgene_69792)

  • For your References section:

    Regulation of the Rac GTPase pathway by the multifunctional Rho GEF Pebble is essential for mesoderm migration in the Drosophila gastrula. van Impel A, Schumacher S, Draga M, Herz HM, Grosshans J, Muller HA. Development. 2009 Mar;136(5):813-22. doi: 10.1242/dev.026203. Epub 2009 Feb 11. 10.1242/dev.026203 PubMed 19176590
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