pHL 1477
(Plasmid
#69787)
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PurposeKanR with FRT sites + ptet::OmpC::T7RNAP::T1 terminator + ColE
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69787 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneColE
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Modifications to backboneFRT sites
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameT7RNAP
- Promoter pTet
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Tag
/ Fusion Protein
- OmpC leader (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer TETp-fwd
- 3′ sequencing primer T1ter-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHL 1477 was a gift from Han Lim (Addgene plasmid # 69787 ; http://n2t.net/addgene:69787 ; RRID:Addgene_69787) -
For your References section:
Horizontal transfer of DNA methylation patterns into bacterial chromosomes. Shin JE, Lin C, Lim HN. Nucleic Acids Res. 2016 Apr 15. pii: gkw230. 10.1093/nar/gkw230 PubMed 27084942