Ths pUAST-HA
(Plasmid #69769)

• Purpose: Thisbe (FGF8-like-1) Ligand in P element-based pUAST vector for Gal4-regulated expression in Drosophila.
• Depositing Lab: Arno Muller
• Publication: Klingseisen et al Development. 2009 Jul;136(14):2393-402. doi: 10.1242/dev.035451. Epub 2009 Jun 10.

Backbone

• Vector backbone: pUAST-HA
• Backbone manufacturer: ?
• Backbone size w/o insert (bp): 8900
• Vector type: Insect Expression ; P element-based pUAST vector for Gal4-regulated expression in Drosophila.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: LB
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Thisbe (FGF8like-1)
• Alt name: Ths, FGF8like-1 ligand
• Species: D. melanogaster (fly)
• Insert Size (bp): 2247
• Mutation: Encodes amino acids 1-739 of Thisbe (NP_610701.2) with H155 insertion
• Promoter: hsp70 promoter
• Tag / Fusion Protein:
  • HA-Tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ? (unknown if destroyed)
• 3′ cloning site: ? (unknown if destroyed)
• 5′ sequencing primer: pUAST for

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Anna Klingseisen, Alumni

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry