Ths pUAST-HA
(Plasmid
#69769)
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PurposeThisbe (FGF8-like-1) Ligand in P element-based pUAST vector for Gal4-regulated expression in Drosophila.
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 69769 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUAST-HA
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Backbone manufacturer?
- Backbone size w/o insert (bp) 8900
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Vector typeInsect Expression ; P element-based pUAST vector for Gal4-regulated expression in Drosophila.
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsLB
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameThisbe (FGF8like-1)
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Alt nameThs, FGF8like-1 ligand
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SpeciesD. melanogaster (fly)
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Insert Size (bp)2247
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MutationEncodes amino acids 1-739 of Thisbe (NP_610701.2) with H155 insertion
- Promoter hsp70 promoter
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Tag
/ Fusion Protein
- HA-Tag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ? (unknown if destroyed)
- 3′ cloning site ? (unknown if destroyed)
- 5′ sequencing primer pUAST for (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAnna Klingseisen, Alumni
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ths pUAST-HA was a gift from Arno Muller (Addgene plasmid # 69769 ; http://n2t.net/addgene:69769 ; RRID:Addgene_69769) -
For your References section:
Differential and overlapping functions of two closely related Drosophila FGF8-like growth factors in mesoderm development. Klingseisen A, Clark IB, Gryzik T, Muller HA. Development. 2009 Jul;136(14):2393-402. doi: 10.1242/dev.035451. Epub 2009 Jun 10. 10.1242/dev.035451 PubMed 19515694