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Addgene

Beta1-GFP pFB-Neo
(Plasmid #69767)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69767 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFB-Neo
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 6600
  • Total vector size (bp) 10300
  • Vector type
    Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Beta1 integrin
  • Alt name
    ITGB1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3700
  • GenBank ID
    NM_002211.3
  • Entrez Gene
    ITGB1 (a.k.a. CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB)
  • Promoter IRES
  • Tag / Fusion Protein
    • GFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer 5´Retro-GGCTGCCGACCCCGGGGGTGG-3´ (bp2008–2028)
  • 3′ sequencing primer 5´-GCCAGGTTTCCGGGCCCTCAC-3´ (bp2147–2127)
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Beta1-GFP pFB-Neo was a gift from Martin Humphries (Addgene plasmid # 69767 ; http://n2t.net/addgene:69767 ; RRID:Addgene_69767)

  • For your References section:

    Quantification of integrin receptor agonism by fluorescence lifetime imaging. Parsons M, Messent AJ, Humphries JD, Deakin NO, Humphries MJ. J Cell Sci. 2008 Feb 1;121(Pt 3):265-71. doi: 10.1242/jcs.018440. 10.1242/jcs.018440 PubMed 18216331
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