Human Lentiviral CRISPR Library v1
(Pooled Library #69763)

• Purpose:

  Genome-scale sgRNA screening library targeting 20,121 human protein-coding genes

• Vector Backbone:

  pLB (Plasmid #11619) - does not express Cas9

• Depositing Labs: Haoquan Wu
• Publication: Ma et al Cell Rep. 2015 Jul 28;12(4):673-83. doi: 10.1016/j.celrep.2015.06.049. Epub 2015 Jul 16.

Library Details

• Species: Human
• Genes targeted: 20,121
• gRNAs: 77,406
• Lentiviral Generation: 3rd

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

• Volume: ∼20 µL
• Concentration: 50 ng/µL

Resource Information

• Depositor Data:
  • Library Composition (genes and target sequences) and Diversity Representation (read counts) (XLSX, 5.8 MB)

Terms and Licenses

Academic/Nonprofit Terms:

• UBMTA

Industry Terms:

• Not Available to Industry

Trademarks:

• Zeocin® is an InvivoGen trademark.

Depositor Comments

This sgRNA library was synthesized as an oligo pool, with 77,406 unique sgRNAs covering 20,121 genes in the human genome (see attached supplemental file for genes and target sequences).

To ensure complete gene disruption, the target sites were chosen in a region close to the translation initiation site, and most genes were covered four times. It is noteworthy that the sgRNAs start with either A or G, because our previous study showed that the preferred transcription initiation nucleotide for the U6 promoter can be either A or G (Ma et al., 2014).

The sgRNA oligo pool was PCR amplified and cloned into the lentiviral vector, pLB. To test its quality, we PCR amplified the library and analyzed the amplicons with deep sequencing. The results showed that at least 90% of the sgRNAs were retained in the plasmid library (see attached supplemental file for sequencing read counts).

For more information about library use and screening, please see the associated publication (Ma et al. Cell Reports 2015, PMID: 26190106).