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Addgene

pKG427
(Plasmid #69754)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69754 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIC242
  • Backbone manufacturer
    Arshad Desai (Addgene plasmid# 44432)
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DSN1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1083
  • Mutation
    all K to R mutations
  • Entrez Gene
    DSN1 (a.k.a. C20orf172, KNL3, MIS13, dJ469A13.2, hKNL-3)
  • Tag / Fusion Protein
    • eGFP-TEV-S (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer pBABE 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKG427 was a gift from Iain Cheeseman (Addgene plasmid # 69754 ; http://n2t.net/addgene:69754 ; RRID:Addgene_69754)

  • For your References section:

    CDK-dependent phosphorylation and nuclear exclusion coordinately control kinetochore assembly state. Gascoigne KE, Cheeseman IM. J Cell Biol. 2013 Apr 1;201(1):23-32. doi: 10.1083/jcb.201301006. Epub 2013 Mar 25. 10.1083/jcb.201301006 PubMed 23530067
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