pIC247
(Plasmid #69741)

• Purpose: Retroviral expression of Borealin
• Depositing Lab: Iain Cheeseman
• Publication: Cheeseman Lab Plasmids (unpublished)

Backbone

• Vector backbone: pJAG98 (pBABEblast)
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Borealin
• Alt name: IMAGE 2959649
• Species: H. sapiens (human)
• Insert Size (bp): 843
• Entrez Gene: CDCA8 (a.k.a. BOR, BOREALIN, DasraB, MESRGP)
• Tag / Fusion Protein:
  • eYFP-TEV-S (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: pBABE 3'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry