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Addgene

pIC301
(Plasmid #69732)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69732 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIC242
  • Backbone manufacturer
    Arshad Desai (Addgene plasmid# 44432)
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bub1
  • Alt name
    BUB1 isoform 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3198
  • GenBank ID
    NM_004336.4 NP_004327.1
  • Entrez Gene
    BUB1 (a.k.a. BUB1A, BUB1L, hBUB1)
  • Tag / Fusion Protein
    • eGFP-TEV-S (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site SacII (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer pBABE 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIC301 was a gift from Iain Cheeseman (Addgene plasmid # 69732 ; http://n2t.net/addgene:69732 ; RRID:Addgene_69732)
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