MAP3K15-TAP
(Plasmid #69728)

• Purpose: Express tandem tagged MAP3K15 in cells
• Depositing Lab: Daniel Liebler
• Publication: ASK_PLASMIDS (unpublished)

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Life Technologies
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 9427
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Apoptosis signal-regulating kinase 3
• Alt name: ASK3
• Alt name: MAP3K15
• Species: H. sapiens (human)
• Insert Size (bp): 3999
• Entrez Gene: MAP3K15 (a.k.a. ASK3, bA723P2.3)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA-Myc tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Partial clone obtained from William Hahn & David Root (Addgene plasmid # 23499)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry