MAP3K6-TAP
(Plasmid #69727)

• Purpose: Express tandem tagged MAP3K6 in cells
• Depositing Lab: Daniel Liebler
• Publication: ASK_PLASMIDS (unpublished)

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Life Technologies
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 9358
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Apoptosis signal-regulating kinase 2
• Alt name: MAP3K6
• Alt name: ASK2
• Species: H. sapiens (human)
• Insert Size (bp): 3930
• Entrez Gene: MAP3K6 (a.k.a. ASK2, MAPKKK6, MEKK6)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA-V5 tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Partial clone obtained from William Hahn & David Root (Addgene plasmid # 23853)
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry