-
Purpose(Empty Backbone) Fluorescent reporter of Agrobacterium rhizogenes mediated transformed roots
-
Depositing Labs
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69709 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepCAMBIA 1305.1
-
Backbone manufacturerCAMBIA, www.cambia.org
- Backbone size (bp) 10701
-
Modifications to backboneDsRed2 gene was inserted in place of the GUSplus reporter gene
-
Vector typePlant Expression
-
Selectable markersHygromycin
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13F
- 3′ sequencing primer M13pUC-Rev (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pHairyRed was a gift from Brett Ferguson & Peter Gresshoff (Addgene plasmid # 69709 ; http://n2t.net/addgene:69709 ; RRID:Addgene_69709) -
For your References section:
pHairyRed: a novel binary vector containing the DsRed2 reporter gene for visual selection of transgenic hairy roots. Lin MH, Gresshoff PM, Indrasumunar A, Ferguson BJ. Mol Plant. 2011 May;4(3):537-45. doi: 10.1093/mp/ssq084. Epub 2011 Feb 15. 10.1093/mp/ssq084 PubMed 21324970
Map uploaded by the depositor.
