MP-S2
(Plasmid #69691)

• Purpose: Untargetted Bacterial Mutagenesis
• Depositing Lab: David Liu
• Publication: Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.

Backbone

• Vector backbone: CloDF13
• Backbone manufacturer: David Liu Lab
• Backbone size w/o insert (bp): 2992
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Turbo
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dnaQ926 AAG(Y127I-H136L)
• Promoter: E. coli PBAD

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: pBAD-F
• 3′ sequencing primer: CAT-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.