MP-J
(Plasmid
#69647)
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PurposeUntargetted Bacterial Mutagenesis
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 69647 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCloDF13
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Backbone manufacturerDavid Liu Lab
- Backbone size w/o insert (bp) 2992
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Turbo
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerpsD12
- Promoter E. coli PBAD
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer pBAD-F
- 3′ sequencing primer CAT-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MP-J was a gift from David Liu (Addgene plasmid # 69647 ; http://n2t.net/addgene:69647 ; RRID:Addgene_69647) -
For your References section:
Development of potent in vivo mutagenesis plasmids with broad mutational spectra. Badran AH, Liu DR. Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425. 10.1038/ncomms9425 PubMed 26443021