cpVenus-Gγ2
(Plasmid #69626)

• Purpose: The heterotrimeric G-protein subunit, Gγ2, tagged with circular permutated Venus (cp173V).
• Depositing Lab: Dorus Gadella
• Publication: van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. doi: 10.1371/journal.pone.0146789. eCollection 2016.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 4930
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cp173Venus-Ggamma2
• Species: Synthetic
• Insert Size (bp): 966
• Entrez Gene: GNG2 (a.k.a. HG3F1)
• Promoter: CMV
• Tag / Fusion Protein:
  • ciruclar permutated Venus (cp173V) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This plasmid is described for the first time in Adjobo-Hermans et al. (2011) BMC Biol. 9:32. doi: 10.1186/1741-7007-9-32. (https://www.ncbi.nlm.nih.gov/pubmed/21619590)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry