Gβ-2A-cpV-Gγ2-IRES-Gαi3-mTq2
(Plasmid #69625)

• Purpose: A FRET sensor for Galphai3 activation, encoded on a single plasmid.
• Depositing Lab: Dorus Gadella
• Publication: van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. doi: 10.1371/journal.pone.0146789. eCollection 2016.

Backbone

• Vector backbone: Clontech-style N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 8445
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Gbeta-2A-cpV-Ggamma2
• Species: Synthetic
• Insert Size (bp): 2046
• Promoter: CMV
• Tag / Fusion Protein:
  • ciruclar permutated Venus (cp173V)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: IRES-R

Gene/Insert 2

• Gene/Insert name: Galphai3-mTurquoise2
• Species: Synthetic
• Insert Size (bp): 1770
• Promoter: CMV (IRES)
• Tag / Fusion Protein:
  • mTurquoise2

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: IRES-F

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry