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Addgene

Gβ-2A-cpV-Gγ2-IRES-Gαi1-mTq2
(Plasmid #69623)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69623 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    Clontech-style N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 8500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Gbeta-2A-cpV-Ggamma2
  • Species
    Synthetic
  • Insert Size (bp)
    2046
  • Promoter CMV
  • Tag / Fusion Protein
    • ciruclar permutated Venus (cp173V)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Galphai-mTurquoise2
  • Species
    Synthetic
  • Insert Size (bp)
    1770
  • Promoter CMV (IRES)
  • Tag / Fusion Protein
    • mTurquoise2

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Gβ-2A-cpV-Gγ2-IRES-Gαi1-mTq2 was a gift from Dorus Gadella (Addgene plasmid # 69623 ; http://n2t.net/addgene:69623 ; RRID:Addgene_69623)
  • For your References section:

    A New Generation of FRET Sensors for Robust Measurement of Galphai1, Galphai2 and Galphai3 Activation Kinetics in Single Cells. van Unen J, Stumpf AD, Schmid B, Reinhard NR, Hordijk PL, Hoffmann C, Gadella TW Jr, Goedhart J. PLoS One. 2016 Jan 22;11(1):e0146789. doi: 10.1371/journal.pone.0146789. eCollection 2016. PONE-D-15-42389 [pii] PubMed 26799488