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PurposeA FRET sensor for Galphai1 activation, encoded on a single plasmid.
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 69623 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneClontech-style N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 8500
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameGbeta-2A-cpV-Ggamma2
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SpeciesSynthetic
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Insert Size (bp)2046
- Promoter CMV
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Tag
/ Fusion Protein
- ciruclar permutated Venus (cp173V)
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer IRES-R (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameGalphai-mTurquoise2
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SpeciesSynthetic
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Insert Size (bp)1770
- Promoter CMV (IRES)
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Tag
/ Fusion Protein
- mTurquoise2
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer IRES-F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Gβ-2A-cpV-Gγ2-IRES-Gαi1-mTq2 was a gift from Dorus Gadella (Addgene plasmid # 69623 ; http://n2t.net/addgene:69623 ; RRID:Addgene_69623) -
For your References section:
A New Generation of FRET Sensors for Robust Measurement of Galphai1, Galphai2 and Galphai3 Activation Kinetics in Single Cells. van Unen J, Stumpf AD, Schmid B, Reinhard NR, Hordijk PL, Hoffmann C, Gadella TW Jr, Goedhart J. PLoS One. 2016 Jan 22;11(1):e0146789. doi: 10.1371/journal.pone.0146789. eCollection 2016. PONE-D-15-42389 [pii] PubMed 26799488