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PurposeThe heterotrimeric G-protein subunit, Gαi isoform 3, tagged with mTurquoise2
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69622 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1(+)
- Backbone size w/o insert (bp) 5300
- Total vector size (bp) 7141
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGalphai3-mTurquoise2
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SpeciesSynthetic
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Insert Size (bp)1770
- Promoter CMV
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Tag
/ Fusion Protein
- mTurquoise2
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Galphai3-mTurquoise2 was a gift from Dorus Gadella (Addgene plasmid # 69622 ; http://n2t.net/addgene:69622 ; RRID:Addgene_69622) -
For your References section:
A New Generation of FRET Sensors for Robust Measurement of Galphai1, Galphai2 and Galphai3 Activation Kinetics in Single Cells. van Unen J, Stumpf AD, Schmid B, Reinhard NR, Hordijk PL, Hoffmann C, Gadella TW Jr, Goedhart J. PLoS One. 2016 Jan 22;11(1):e0146789. doi: 10.1371/journal.pone.0146789. eCollection 2016. PONE-D-15-42389 [pii] PubMed 26799488
