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Addgene

pAAV-EF1a-flex-TVA
(Plasmid #69618)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69618 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 5604
  • Total vector size (bp) 6780
  • Vector type
    Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Avian sarcoma and leukemia virus receptor 950
  • Alt name
    TVA950
  • Species
    Synthetic; quail
  • Insert Size (bp)
    1145
  • GenBank ID
    AY531260.1
  • Entrez Gene
    CD320 (a.k.a. TVA)
  • Promoter EF1a
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (destroyed during cloning)
  • 3′ cloning site SpeI (destroyed during cloning)
  • 5′ sequencing primer EF1a forward
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    John A. T. Young

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-EF1a-flex-TVA was a gift from Naoshige Uchida (Addgene plasmid # 69618 ; http://n2t.net/addgene:69618 ; RRID:Addgene_69618)

  • For your References section:

    Dopamine neurons projecting to the posterior striatum form an anatomically distinct subclass. Menegas W, Bergan JF, Ogawa SK, Isogai Y, Umadevi Venkataraju K, Osten P, Uchida N, Watabe-Uchida M. Elife. 2015 Aug 31;4. doi: 10.7554/eLife.10032. 10.7554/eLife.10032 PubMed 26322384
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