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Purpose(Empty Backbone) To express gene strongly with AAV
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69616 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAAV
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Backbone manufacturerStratagene
- Backbone size (bp) 5604
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Modifications to backboneCA promoter was added
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Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer XpAAVE-F (GCTAACCATGTTCATGCCTTC) (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-CA was a gift from Naoshige Uchida (Addgene plasmid # 69616 ; http://n2t.net/addgene:69616 ; RRID:Addgene_69616) -
For your References section:
Dopamine neurons projecting to the posterior striatum form an anatomically distinct subclass. Menegas W, Bergan JF, Ogawa SK, Isogai Y, Umadevi Venkataraju K, Osten P, Uchida N, Watabe-Uchida M. Elife. 2015 Aug 31;4. doi: 10.7554/eLife.10032. 10.7554/eLife.10032 PubMed 26322384
