pGM1202
(Plasmid #69615)

• Purpose: inducible gene expression in Streptomyces, C-terminal His-tag encoding sequence
• Depositing Lab: Günther Muth
• Publication: Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(21):9067-9080. doi: 10.1007/s00253-018-9334-5. Epub 2018 Sep 6.

Backbone

• Vector backbone: pSG5
• Backbone size w/o insert (bp): 2140

Growth in Bacteria

• Bacterial Resistance(s): Apramycin, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: replicates also in Streptomyces (below 35°C)
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: aac
• Alt name: aac(3)IV
• Species: E. coli
• Insert Size (bp): 803

Gene/Insert 2

• Gene/Insert name: tsr
• Alt name: thiostrepton resistance
• Species: Streptomyces azureus
• Insert Size (bp): 809
• GenBank ID: X02392

Gene/Insert 3

• Gene/Insert name: rep
• Species: Streptomyces ghanaensis
• Insert Size (bp): 1442

Gene/Insert 4

• Gene/Insert name: sso
• Alt name: single stranded origin
• Species: Streptomycves ghanaensis
• Insert Size (bp): 300

Gene/Insert 5

• Gene/Insert name: TipA promoter
• Alt name: P_tipA
• Species: Streptomyces lividans
• Insert Size (bp): 100

Gene/Insert 6

• Gene/Insert name: dso
• Alt name: double-stranded origin

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry