pGM190
(Plasmid #69614)

• Purpose: inducible gene expression in Streptomyces
• Depositing Lab: Günther Muth
• Publication: Reuther et al Mol Microbiol. 2006 Jul;61(2):436-46. doi: 10.1111/j.1365-2958.2006.05258.x. Epub 2006 Jun 15.

Backbone

• Vector backbone: pSG5
• Backbone size w/o insert (bp): 2140
• Total vector size (bp): 6874
• Vector type: Bacterial Expression ; Streptomyces- E. coli shuttle vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Replicates also in Streptomyces strains ( below 35°C), selection kanamycin or thiostrepton
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: aphII
• Alt name: kan, neo
• Species: Transposon Tn5
• Insert Size (bp): 794

Gene/Insert 2

• Gene/Insert name: tsr
• Alt name: thiostrepton resistance
• Species: Streptomyces azureus
• Insert Size (bp): 809
• GenBank ID: X02392

Gene/Insert 3

• Gene/Insert name: rep
• Species: Streptomyces ghanaensis
• Insert Size (bp): 1442

Gene/Insert 4

• Gene/Insert name: sso
• Alt name: single stranded origin
• Species: Streptomycves ghanaensis
• Insert Size (bp): 300

Gene/Insert 5

• Gene/Insert name: TipA promoter
• Alt name: P_tipA
• Species: Streptomyces lividans
• Insert Size (bp): 100

Gene/Insert 6

• Gene/Insert name: dso
• Alt name: double-stranded origin
• Species: unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Stable replicating, multi copy shuttle vector for thiostrepton induced gene expression in Streptomyces
temperature sensitive replication (G. Muth, B. Nußbaumer, W. Wohlleben and A. Pühler (1989) A vector system with temperature-sensitive replication for gene disruption and mutational cloning in streptomycetes. Mol. Gen. Genet. 219, 341-348 )
http://www.uni-tuebingen.de/en/faculties/faculty-of-science/departments/inter-faculty-institutes-and-centres/imit/units/mikrobiologiebiotechnologie/work-groups/muth/plasmids.html