CYP2C19
(Plasmid #69606)

• Purpose: For protein expression of CYP2C19
• Depositing Lab: Joyce Goldstein
• Publication: Goldstein CYP2C9 and CYP2C9 in pOri+ for bacterial expression (unpublished)

Backbone

• Vector backbone: pCW
• Backbone size w/o insert (bp): 5500
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CYP2C19
• Species: H. sapiens (human)
• Mutation: deletion of AA1-8 and mutation I331V
• GenBank ID: NM_000769.2
• Entrez Gene: CYP2C19 (a.k.a. CPCJ, CYP2C, CYPIIC17, CYPIIC19, P450C2C, P450IIC19)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: pCW-SEQ-fwd (5'ACATCGTATAACGTTACTGG-3')
• 3′ sequencing primer: pCW-SEQ-rev (5'-CTTTCGTCTTCAAGCAGATCTG-3')

Resource Information

• Supplemental Documents:
  • sequence of modified cDNA
• Addgene Notes:
  • Feedback from Dr. James Behrendorff; University of Copenhagen

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Modification of CYP2C19 for bacterial expression as per Barnes et al. PNAS 88:5597, 1991 [PMID: 1829523]: First 8 amino acids of CYP2C cDNAs replaced with those of bovine 17α hydroxylase (MALLLAVF) to optimize bacterial expression.