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Addgene

paGFP-Omp25
(Plasmid #69598)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69598 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLJM2
  • Backbone manufacturer
    Jason Moffat
  • Backbone size w/o insert (bp) 7327
  • Total vector size (bp) 8164
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    paGFP-Omp25
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    837
  • Mutation
    photoactivatable GFP fused to rat Omp25 C-terminus
  • GenBank ID
    NM_022599.2
  • Entrez Gene
    Synj2bp (a.k.a. Omp25)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer pLJM1 R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    paGFP-Omp25 was a gift from David Sabatini (Addgene plasmid # 69598 ; http://n2t.net/addgene:69598 ; RRID:Addgene_69598)

  • For your References section:

    Stem cells. Asymmetric apportioning of aged mitochondria between daughter cells is required for stemness. Katajisto P, Dohla J, Chaffer CL, Pentinmikko N, Marjanovic N, Iqbal S, Zoncu R, Chen W, Weinberg RA, Sabatini DM. Science. 2015 Apr 17;348(6232):340-3. doi: 10.1126/science.1260384. Epub 2015 Apr 2. 10.1126/science.1260384 PubMed 25837514
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