pBlueRIP.OVA
(Plasmid
#69597)
-
Purposecontains soluble form of ovalbumin (OVA) cDNA in pBlueRIP (used to generate RIP-OVAhi and RIP-OVA lo mice)
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 69597 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepBlueRIP
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameOVA
-
Alt namemembrane bound form of ovalbumin (OVA)
-
SpeciesG. gallus (chicken)
-
Insert Size (bp)1900
-
Entrez GeneOVAL (a.k.a. OVA, SERPINB14)
- Promoter rat insulin promoter (RIP)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer pBluescript-KS
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBlueRIP.OVA was a gift from Francis R. Carbone (Addgene plasmid # 69597 ; http://n2t.net/addgene:69597 ; RRID:Addgene_69597) -
For your References section:
Induction of autoimmune diabetes by oral administration of autoantigen. Blanas E, Carbone FR, Allison J, Miller JF, Heath WR. Science. 1996 Dec 6;274(5293):1707-9. PubMed 8939860