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Addgene

pBlueRIP.cOVA
(Plasmid #69595)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69595 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBlueRIP
  • Total vector size (bp) 5200
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    OVA
  • Alt name
    cytoplasmic form of ovalbumin (OVA)
  • Species
    G. gallus (chicken)
  • Entrez Gene
    OVAL (a.k.a. OVA, SERPINB14)
  • Promoter rat insulin promoter (RIP)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pBluescript-KS
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ApaI/NotI removes a 2.3 kb RIP/cOVA/polyA insert. The rat insulin promoter is located at bp# 2794-2083

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBlueRIP.cOVA was a gift from Francis R. Carbone (Addgene plasmid # 69595 ; http://n2t.net/addgene:69595 ; RRID:Addgene_69595)

  • For your References section:

    Constitutive class I-restricted exogenous presentation of self antigens in vivo. Kurts C, Heath WR, Carbone FR, Allison J, Miller JF, Kosaka H. J Exp Med. 1996 Sep 1;184(3):923-30. PubMed 9064352
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