Skip to main content
Addgene

p3A9cbetaTCR.HSV2.3
(Plasmid #69594)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69594 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    p3A9CbetaTCR
  • Backbone manufacturer
    Carbone Lab, Addgene plasmid 69600
  • Total vector size (bp) 13590
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TCR beta VJ region from clone HSV2.3
  • Alt name
    T cell receptor beta chain
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1000
  • Entrez Gene
    Tcrb (a.k.a. TCRbeta, Tib)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer AATCGATGGCCACTGGTGTGCTT
  • 3′ sequencing primer ATCGATTCTTCCTCAAGGAGGAACCA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ApaI/NotI removes transgenic construct

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3A9cbetaTCR.HSV2.3 was a gift from Francis R. Carbone (Addgene plasmid # 69594 ; http://n2t.net/addgene:69594 ; RRID:Addgene_69594)

  • For your References section:

    Characterization of two TCR transgenic mouse lines specific for herpes simplex virus. Mueller SN, Heath W, McLain JD, Carbone FR, Jones CM. Immunol Cell Biol. 2002 Apr;80(2):156-63. 10.1046/j.1440-1711.2002.01071.x PubMed 11940116
Related items:
Commonly requested with: