pCRII-1.1-1.10a
(Plasmid
#69589)
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PurposeOT-II TCR a chain cDNA in pCRII
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69589 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCRII
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTCR alpha clone 1.1
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Alt nameT cell receptor alpha chain
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SpeciesM. musculus (mouse)
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Insert Size (bp)800
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Entrez GeneTcra (a.k.a. Tcralpha)
Cloning Information
- Cloning method TOPO Cloning
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCRII-1.1-1.10a was a gift from Francis R. Carbone (Addgene plasmid # 69589 ; http://n2t.net/addgene:69589 ; RRID:Addgene_69589) -
For your References section:
Defective TCR expression in transgenic mice constructed using cDNA-based alpha- and beta-chain genes under the control of heterologous regulatory elements. Barnden MJ, Allison J, Heath WR, Carbone FR. Immunol Cell Biol. 1998 Feb;76(1):34-40. 10.1046/j.1440-1711.1998.00709.x PubMed 9553774
Map uploaded by the depositor.
