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Addgene

pRRLNeo-pEF1a-p53ashL344A-mCerulean
(Plasmid #69580)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69580 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRRL
  • Backbone size w/o insert (bp) 7429
  • Total vector size (bp) 10565
  • Modifications to backbone
    neomycin resistance
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    p53-L344A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1179
  • Mutation
    p53 L344A mutation that prevents tetramerization and 7 silent mutations in p53 DNA to prevent targeting by shRNA at positions C777T, T778A, C779G, A781T, G782C, T786A, T789C
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
  • Promoter EF1alpha
  • Tag / Fusion Protein
    • mCerulean (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This mutant will dimerize, but not tetramerize. The silent mutations prevent targeting by shRNA p53 published in Brummelkamp et al Science. 2002;296:550–3.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRRLNeo-pEF1a-p53ashL344A-mCerulean was a gift from Galit Lahav (Addgene plasmid # 69580 ; http://n2t.net/addgene:69580 ; RRID:Addgene_69580)
  • For your References section:

    Activation and control of p53 tetramerization in individual living cells. Gaglia G, Guan Y, Shah JV, Lahav G. Proc Natl Acad Sci U S A. 2013 Sep 17;110(38):15497-501. doi: 10.1073/pnas.1311126110. Epub 2013 Sep 4. 10.1073/pnas.1311126110 PubMed 24006363