pB3.P11
(Plasmid #69577)

• Purpose: OT-I TCR beta chain cDNA in pCRScript
• Depositing Lab: Francis R. Carbone
• Publication: Hogquist et al Cell. 1994 Jan 14;76(1):17-27.

Backbone

• Vector backbone: pCR-Script SK+
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 4000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TCR beta cDNA clone B3
• Alt name: T cell receptor beta chain
• Species: M. musculus (mouse)
• Insert Size (bp): 1000
• Entrez Gene: Tcrb (a.k.a. TCRbeta, Tib)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry