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Addgene

p42.3A7
(Plasmid #69575)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69575 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR1000
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TCR alpha clone 149.42
  • Alt name
    T cell receptor alpha chain
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    830
  • Entrez Gene
    Tcra (a.k.a. Tcralpha)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer M13pUC-fwd
  • 3′ sequencing primer M13pUC-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p42.3A7 was a gift from Francis R. Carbone (Addgene plasmid # 69575 ; http://n2t.net/addgene:69575 ; RRID:Addgene_69575)

  • For your References section:

    T cell receptor antagonist peptides induce positive selection. Hogquist KA, Jameson SC, Heath WR, Howard JL, Bevan MJ, Carbone FR. Cell. 1994 Jan 14;76(1):17-27. 0092-8674(94)90169-4 [pii] PubMed 8287475
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