pKS913.CB18.31
(Plasmid #69569)

• Purpose: TCR beta chain genomic transgenic construct for creating OT-1 mice
• Depositing Lab: Francis R. Carbone
• Publication: Carbone et al Int Immunol. 1992 Aug;4(8):861-7.

Backbone

• Vector backbone: pBluescript KS
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Modifications to backbone: TCR beta chain enhancer element cloned into the BamHI site of the plasmid Bluescript pKS
• Vector type: Bacterial Expression, Mouse Targeting

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TCR beta
• Alt name: Genomic T cell receptor beta chain from CTL clone B3
• Species: M. musculus (mouse)
• Insert Size (bp): 14500
• Entrez Gene: Tcrb (a.k.a. TCRbeta, Tib)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: M13pUC-fwd
• 3′ sequencing primer: M13pUC-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert is a 14.5 kb fragment containing the TCR beta-chain coding and promoter regions but lacking the 3' TCR beta-chain enhancer.