pET23a-HsRPS27a
(Plasmid
#69561)
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PurposeBacterial expression; in vitro transcription/translation (T7)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 69561 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-23a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 3626
- Total vector size (bp) 4097
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameRPS27A
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Alt nameUBA80
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Alt nameUbiquitin A-80
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Alt nameUBCEP1
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SpeciesH. sapiens (human)
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Insert Size (bp)471
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GenBank IDNM_001135592.2
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Entrez GeneRPS27A (a.k.a. CEP80, HEL112, S27A, UBA80, UBC, UBCEP1, UBCEP80, eS31)
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET23a-HsRPS27a was a gift from Jorge Eduardo Azevedo (Addgene plasmid # 69561 ; http://n2t.net/addgene:69561 ; RRID:Addgene_69561) -
For your References section:
The de novo synthesis of ubiquitin: identification of deubiquitinases acting on ubiquitin precursors. Grou CP, Pinto MP, Mendes AV, Domingues P, Azevedo JE. Sci Rep. 2015 Aug 3;5:12836. doi: 10.1038/srep12836. 10.1038/srep12836 PubMed 26235645