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pET23a-MmUBB(SnaBI)
(Plasmid #69558)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69558 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-23a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3626
  • Total vector size (bp) 4544
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    UBB
  • Alt name
    polyubiquitin B
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    918
  • Mutation
    The two last codons (TATTAA; encoding the last tyrosine and the stop codon) were replaced by TACGTA by site-directed mutagenesis to generate a SnaBI restriction site.
  • GenBank ID
    NM_011664.3
  • Entrez Gene
    Ubb (a.k.a. AL033289, Rps27a, Uba52, Ubb2, Ubc)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid digestion with the restriction enzyme SnaBI generates a linear DNA fragment that allows in vitro synthesis of a stopless UBB protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET23a-MmUBB(SnaBI) was a gift from Jorge Eduardo Azevedo (Addgene plasmid # 69558 ; http://n2t.net/addgene:69558 ; RRID:Addgene_69558)

  • For your References section:

    The de novo synthesis of ubiquitin: identification of deubiquitinases acting on ubiquitin precursors. Grou CP, Pinto MP, Mendes AV, Domingues P, Azevedo JE. Sci Rep. 2015 Aug 3;5:12836. doi: 10.1038/srep12836. 10.1038/srep12836 PubMed 26235645
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