tetO-GFPnls-mPGK-rtTA-IRES-Puro
(Plasmid
#69545)
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PurposeTet-On system expressing GFP tagged with 2x nuclear localization signals.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69545 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRRL
- Total vector size (bp) 10050
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameeGFP
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Alt nameEnhanced green fluorescent protein
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SpeciesSynthetic
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Tags
/ Fusion Proteins
- 2x nuclear localization sequence (C terminal on insert)
- T2A "skipping" peptide (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
tetO-GFPnls-mPGK-rtTA-IRES-Puro was a gift from Charles Gersbach (Addgene plasmid # 69545 ; http://n2t.net/addgene:69545 ; RRID:Addgene_69545)
Map uploaded by the depositor.
