GG-EBNA-OCT4
(Plasmid
#69537)
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PurposeEpisomal plasmid encoding 5 gRNAS targeting human OCT4 promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69537 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneGGdest
- Total vector size (bp) 10941
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Vector typeCRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name5 concatenated gRNA transcriptional cassettes targeting OCT4
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gRNA/shRNA sequencehuman OCT4 promoter
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byEBNA-ori fragment used from Addgene Plasmid #27078.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GG-EBNA-OCT4 was a gift from Timo Otonkoski (Addgene plasmid # 69537 ; http://n2t.net/addgene:69537 ; RRID:Addgene_69537) -
For your References section:
Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation. Balboa D, Weltner J, Eurola S, Trokovic R, Wartiovaara K, Otonkoski T. Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 10.1016/j.stemcr.2015.08.001. 10.1016/j.stemcr.2015.08.001 PubMed 26352799
