CFP-GIV-CT FA
(Plasmid #69533)

• Purpose: Mammalian expression of The CFP-tagged GIV-CT-FA biosensors
• Depositing Lab: Pradipta Ghosh
• Publication: Midde et al Proc Natl Acad Sci U S A. 2015 Mar 3;112(9):E937-46. doi: 10.1073/pnas.1420140112. Epub 2015 Feb 17.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 5848
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GIV
• Alt name: GIV-CT-F1685A
• Species: H. sapiens (human)
• Insert Size (bp): 420
• Mutation: C terminal amino acids S1660-S1870 of human GIV with F1685A mutation.
• GenBank ID: BAE44387.1 AB201172.1
• Entrez Gene: CCDC88A (a.k.a. APE, GIRDIN, GIV, GRDN, HkRP1, KIAA1212, PEHO, PEHOL)
• Promoter: CMV
• Tag / Fusion Protein:
  • CFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn1 (not destroyed)
• 3′ cloning site: Not1 (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry