rEc.13.delA
(Bacterial strain
#69495)
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PurposeRecoded strain from MG1655, prfA deleted
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Bacterial Strain | 69495 | Bacteria in agar stab | 1 | $85 |
Backbone
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Vector backboneStrain
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)MG1655
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Growth instructionsDo not grow over 34 oC. 42 oC activates lambda red cassette expression.
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameStrain
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
rEc.13.delA was a gift from Michael Jewett (Addgene plasmid # 69495) -
For your References section:
Cell-free protein synthesis from a release factor 1 deficient Escherichia coli activates efficient and multiple site-specific nonstandard amino acid incorporation. Hong SH, Ntai I, Haimovich AD, Kelleher NL, Isaacs FJ, Jewett MC. ACS Synth Biol. 2014 Jun 20;3(6):398-409. doi: 10.1021/sb400140t. Epub 2014 Jan 2. 10.1021/sb400140t PubMed 24328168