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Addgene

Cherry-SRBC
(Plasmid #69492)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69492 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mCherryC1
  • Backbone manufacturer
    Clontech
  • Modifications to backbone
    Cherry replaced GFP in the pEGFP-C1 vector
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SRBC
  • Alt name
    Cavin3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    784
  • Entrez Gene
    Cavin3 (a.k.a. 3110015B12Rik, 6330514M23Rik, Prkcdbp, SRBC)
  • Promoter CMV
  • Tag / Fusion Protein
    • Cherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer Cherry Forward
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cherry-SRBC was a gift from Rob Parton (Addgene plasmid # 69492 ; http://n2t.net/addgene:69492 ; RRID:Addgene_69492)

  • For your References section:

    Single-molecule analysis reveals self assembly and nanoscale segregation of two distinct cavin subcomplexes on caveolae. Gambin Y, Ariotti N, McMahon KA, Bastiani M, Sierecki E, Kovtun O, Polinkovsky ME, Magenau A, Jung W, Okano S, Zhou Y, Leneva N, Mureev S, Johnston W, Gaus K, Hancock JF, Collins BM, Alexandrov K, Parton RG. Elife. 2013 Jan 1;3:e01434. doi: 10.7554/eLife.01434. 10.7554/eLife.01434 PubMed 24473072
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