pJW1354
(Plasmid
#69490)
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PurposeGly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology arms
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69490 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCR-Blunt II-TOPO
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Backbone manufacturerInvitrogen
- Total vector size (bp) 3862
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Vector typeCRISPR ; template to generate cassettes for -mediated knock-in
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGSGGGG spacer-Degron-TEV protease site-3x FLAG epitope
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)246
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13 fwd
- 3′ sequencing primer M13 rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJW1354 was a gift from Jordan Ward (Addgene plasmid # 69490 ; http://n2t.net/addgene:69490 ; RRID:Addgene_69490) -
For your References section:
The auxin-inducible degradation (AID) system enables versatile conditional protein depletion in C. elegans. Zhang L, Ward JD, Cheng Z, Dernburg AF. Development. 2015 Nov 9. pii: dev.129635. 10.1242/dev.129635 PubMed 26552885
Map uploaded by the depositor.
